anti mouse igg antibody (Bio-Rad)

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Bio-Rad anti mouse igg antibody
Anti Mouse Igg Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-ovine+igg/Mouse+anti+Bovine%2FOvine+IgG%2FA%2FM/pmc12334472-138-7-20
Average 93 stars, based on 1 article reviews

anti mouse igg antibody - by Bioz Stars, 2026-10

93/100 stars

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Incubation:

Article Title: Identification of a thrombospondin-like immunodominant and phosphorylcholine-containing glycoprotein (GP300) in Dictyocaulus viviparus and related nematodes.
Article Snippet: GP300 is a high molecular weight glycoprotein of the bovine lungworm Dictyocaulus viviparus.. The Nlinked glycans are substituted with phosphorylcholine (PC) giving it immunomodulatory potential.. GP300 is highly immunogenic and its recognition by IgE antibodies is correlated with protection against infection.

Article Title: Impact of specific productivity and operation mode upon the biophysical properties of HIV-1 Gag-based virus-like particles
Article Snippet: .. After washing, immunodetection was performed using an anti-mouse IgG antibody conjugated with a horseradish peroxidase (dilution 1:5000 in PBS 1X) (Bio-Rad Laboratories, Hercules, CA, USA), incubated for 2 h at room temperature, and washed with PBS 0.1% (w/v) Tween-20. .. Protein bands were visualized by incubating the membranes with a ClarityTM Western ECL Substrate solutions for 2–3 min, and scans were taken in a ChemiDoc MP (Bio-Rad Laboratories, Hercules, CA, USA).

Staining:

Imaging:

Article Title: The STING-activating nanofactory relieves T cell exhaustion in Mn-based tumor immunotherapy by regulating mitochondrial dysfunction
Article Snippet: The membrane was treated with western blocking buffer (Beyotime, China) for 1 h on a shaker and then incubated with specific primary antibodies at 4 °C overnight, including β-actin, HIF-1α, STING, phospho-STING, IRF3, phospho-IRF3. .. Next, HRP-conjugated Goat anti-Rabbit IgG or anti-Mouse IgG was used as the secondary antibody, and protein bands were visualized using a ChemiDoc MP Imaging System (Bio-Rad, USA). .. Female BALB/c mice (approximately 6 weeks old) were sourced from the Shanghai Laboratory Animal Center (SLAC, Shanghai, China) and housed in a sterilized, specific pathogen-free (SPF) facility at Tongji University.

other:

Article Title: Autologous bone marrow-derived MSCs engineered to express oFVIII-FLAG engraft in adult sheep and produce an effective increase in plasma FVIII levels
Article Snippet: Sheep plasma samples were serially diluted starting at 1:20 dilution, and antibody binding was detected with anti-ovine IgG : ALP (Bio-Rad Laboratories) or anti-ovine IgM : ALP (Abcam, Cambridge, UK) and p -nitrophenyl-phosphate (Bio-Rad Laboratories).

Concentration Assay:

Article Title: Cytokine profile, differential somatic cell count, and oxidative status of Italian Mediterranean buffalo milk affected by the temperature–humidity index
Article Snippet: Briefly, mouse monoclonal antibodies (mAbs) for ovine IL-1β (Clone 1D4, Bio-Rad Ltd., at a final concentration of 2 μg/mL), and bovine IL-10 (Clone CC318, Bio-Rad Ltd., at a final concentration of 2 μg/mL) were used as capture antibodies. .. The detecting antibodies were represented by the rabbit polyclonal anti-ovine IL-1β Ab (Polyclonal IgG, Bio-Rad Ltd., at a final concentration of 2 μg/mL) and the biotinylated secondary anti-bovine IL-10 mAb (Clone CC320, Bio-Rad Ltd., at a final concentration of 2 μg/mL). .. The samples were compared to a standard curve generated using a 4-parameter logistic fit method based on serial dilutions of recombinant bovine IL-1β protein (Kingfisher Biotech Inc., St. Paul, MN, USA, range 500–0 ng/mL, and R 2 = 0.995), which has 99% homology with buffalo IL-1β protein, as stated by the manufacturer.

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anti mouse igg antibody (Bio-Rad)

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